Contact an Account ManagerComparing the separations of water-soluble vitamins
Summary
Type: HPLC
Application #: 126730
Catalog#: 763234.30 - MN HPLC Column, Nucleoshell RP 18plus, 90A, 2.7um, 3x100mm
Detection:
Conditions: Eluent A: 20 mmol/L KH2PO4 adjusted to pH 3.0
Eluent B: acetonitrile – methanol (30:70, v/v)
Gradient: 0 % B for 2 min, to 30 % B in 1 min, hold for 12 min
Flow rate: 0.25 mL/min
Temperature: 15 °C
Injection volume: 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2014
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